Aurigene's molecular biologists are well versed in cloning and expression of cDNAs encoding chosen molecular targets. High level target protein expression is achieved using bacterial, baculovirus / insect cells, mammalian (transient and stable) or yeast systems. Expression of target proteins in soluble and active form is accomplished by taking advantage of a variety of novel vector and host strains.

Recombinant proteins are purified in large quantities from the suitable expression systems utilizing traditional and affinity chromatography methods. State-of-the-art protein purification instruments such as FPLC are extensively utilized to purify proteins for screening and structural biology studies.

Crystallization studies are performed for target proteins in complex with known ligands as well as Aurigene synthesized / collaborator compounds, supporting the SBDD group in the Lead Generation / Optimization process.