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Cell Biology: Aurigene's Cell Biology includes a collection of primary & established cell lines including cell lines stably expressing molecular drug targets. These cell lines are routinely used for protein expression, establishment of cell-based assays and in vitro validation of the mechanism of action of candidate compounds.


In-vitro ADME/ Tox: The In-vitro ADME/Tox group performs the following screening assays in support of lead generation and optimization efforts.

  Aqueous solubility and stability at various pHs.
  Metabolic stability and intrinsic clearance with microsomes or primary hepatocytes
  Metabolite identification, Phase I and Phase II Metabolism
  Permeability using PAMPA, MDCK and Caco-2
  Plasma protein binding determination
  Cytochrome P450 inhibition
  Cytochrome P450 induction
  Cytotoxicity assay using cell lines or hepatocytes
  Mutagenicity assays including Ames and micronucleus test


In-vivo Biology: Aurigene has an excellent in-house animal facility currently housing rodents. The following activities are performed in support of lead generation and optimization efforts.

  In vivo PK - Pharmacokinetic studies, Tissue distribution studies and Metabolism studies
  Disease/ Efficacy Models - Oncology, Inflammation, Metabolic diseases
  Safety Pharmacology - CNS, Respiratory & GI safety studies
  Toxicology
  Genetic Toxicology    
  AMES test, micronucleus test    
  General Toxicology    
  Acute Toxicity & repeated dose toxicity with toxicokinetics    

 

Pre-Clinical Biology
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